The DNA end resection activities of different mutants have been compared on the same gel. Briefly, 400 nM substrate DNA was incubated with drNurA (4 μM protomer) and drHerA (12 μM protomer) in the presence of 2 mM MgCl 2 and 8 mM MnCl 2. Whenever needed, 1 mM ligand (ADP, ADPNP, or ATP) was added to the reaction system. Products were analyzed on 15% denaturing TBE-PAGE. The same gels were imaged at FAM fluorescent mode (upper) and Cy5 fluorescent mode (lower) separately. The types of the generated products before denaturing have been annotated at the right side of the gel to aid the interpretation of the bands on the gels. Markers were created by mixing different lengths of 3′ FAM or 5′ Cy5 labeled DNA oligos together. dPin, NurA dPin ; 487/8, HerA E487A/E488A ; m, HerA m ; 13, NurA F13A ; 8, NurA W8A ; dN7, drNurAdN7; 53, NurA D53A ; 201, NurA K201A ; 309, NurA R309A ; 96, HerA R96A ; 495, HerA R495A ; 552, HerA R552A
