Heatmap from Scientific Research

Virulence profile of the isolates vs the cultivar panel based on levels of leaf necrosis and chlorosis. Measurements were taken by both visual assessments and by using the LemnaTec, LemnaGrid image analysis software with comparable final results. Isolates were ranked and clustered based on virulence data. Z. tritici isolates highlighted by # were genome sequenced to construct a pangenome. Isolates highlighted by + were also analysed by RNAseq transcriptomics. Wheat cultivar Panorama (highlighted by X) was determined to be equally and fully susceptible to most isolates and was selected as the host genotype for the leaf infection RNAseq. Note the low virulence data for the outgroup of seven isolates against all cultivars is likely to result from these isolates being adapted to causing disease on tetraploid wheat (Durum or Pasta). All data is representative of three infected leaves analysed/interaction from two biological replicate experiments (6 leaves in total).

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