Agrobacterium mediated co-expression of genes encoding AvrPm8-FLAG, AvrPm8_F43Y-FLAG, AvrPm8_F43L-FLAG, AvrPm8-R96STOP-FLAG, or AvrPm8-L77STOP-FLAG with Pm8-HA in N. benthamiana . Leaves were imaged at 5 days post inoculation using a Fusion FX imager system (top panel). Co-infiltrations were performed with a ratio of 4 (effector): 1 (Pm8-HA). Middle panel: quantification of HR intensity in co-expression assay depicted in top panel. Individual datapoints are color coded based on four independent experiments with n = 16. Detection of N. benthamiana expressed AvrPm8 constructs by anti-FLAG western blotting (bottom panel) and total protein Ponceau S staining as a loading control. Black arrows indicate positions of protein size markers.
