Schematic gene model (top) and phenotypes (bottom) of isolates with the identified gain-of-virulence mutations in the AvrPm8 effector gene. The two exons of AvrPm8 are depicted as yellow boxes with gene orientation indicated by an arrow. Start codon, splice sites, and stop codon are indicated. Mutations leading to an amino acid change (blue) or a premature stop codon (red) are indicated by a colored line. For each mutation, the virulence phenotype of a representative isolate on the Pm8 transgenic line Pm8#34 is depicted. Susceptible cultivar “Bobwhite” serves as a control. The phenotype of the avirulent isolate ISR_7 is shown as comparison (middle). Additional phenotyping data for each isolate including phenotypes on the near-isogenic line “Kavkaz/4*Federation” and an independent Pm8 transgenic line Pm8#12 are depicted in Additional file 1 : Fig. S5. For two mutations (K28STOP and spliceAT-AG), we were unable to find a representative isolate in our living B.g. tritici collection.
