Gel from Scientific Research

Major proteins in isolated membrane ghosts and in supernatants after centrifugation of membrane ghosts of normal and sickle erythrocytes. The samples were separated by SDS-PAGE, transferred to nitrocellulose membrane, and stained with ponceau S. Lane 1: protein marker; lanes 2-4: isolated membrane ghosts of the normal erythrocytes from three healthy subjects; lanes 5-7: isolated membrane ghosts of the sickle erythrocytes from three sickle cell anemia patients; lanes 8-12: the supernatants after 1st, 4th, and 7th centrifugation of membrane ghosts of normal erythrocytes from two healthy subjects; lanes 13-18: the supernatants after 1st, 4th, and 7th centrifugation of membrane ghosts of sickle erythrocytes from two patients.

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