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Scheme for antibiotic and microbiota colonization. WT mice were treated with broad-spectrum antibiotics for 3 days, transferred with either WT or γδ -/- microbiota 2 days later and continuously fed OVA (or only water as control) starting 3 days following microbiota transfer and ending 5 days later. Mice were then immunized with OVA/CFA 2 days after the last dose of OVA.
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