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Microbiota was depleted with a combination of 4 antibiotics (ABX) in the drinking water for 3 days and 1 day later, microbiota from WT and / mice were swapped. To assess oral tolerance, 3 days later, half of each group were fed OVA in the drinking water for 5 days. OVA continuous feeding was stopped and 2 days later mice were immunized with OVA/CFA. In an independent cohort, the immune responses were characterized 17 days post colonization.

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