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Microbiota was depleted with a combination of 4 antibiotics (ABX) in the drinking water for 3 days, and control mice did not receive antibiotics ( n = 10 mice each). Half of each group were then fed OVA in the drinking water for 5 days and antibiotics were maintained during the OVA feeding period. Antibiotics and OVA were stopped and 2 days later mice were immunized with OVA/CFA. Responsiveness to OVA was measured by splenocyte proliferation upon 100 g/mL of OVA stimulation. Data are mean SEM; n =45 mice/group; one-way ANOVA.
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