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Western blot analysis of TREM2 isoforms C-terminus (FLAG-tagged) and N-terminus (HA-tagged) in cell extracts (left panel) and media (right panel) of HEK-293T transfected with tagged TREM2 isoforms. The FLAG and HA antibodies were used to detect the C- and N-terminus, respectively. beta-Actin and Ponceau staining were used as loading controls for cell extracts and media, respectively.

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