Transmission electron microscopy (TEM) analysis of Era and CQ(1) effects on lysosome morphology in WT and SLC7A11KO HT1080 cells (scale bar, 100 nm;n= 18–35 images). Unless otherwise indicated, cells were co-treated with Fer-1 (2 μM) to prevent ferroptosis. Data are means ± SEMs and dots are indicated samples from ≥3 biological repeats.∗∗∗p< 0.001,∗∗p< 0.01,∗p< 0.05, NS,p> 0.05. See alsoFigure S5.
