Line Plot from Scientific Research

Size-exclusion chromatography profiles of purified recombinant VNp-mNG and VNp-LZ-mNG proteins (inset) confirmed that introduction of a leucine zipper (LZ) motif to the VNp-mNeongreen (mNG) fusion induced stable dimer formation. Each fusion protein as well as protein standards (29 kDa carbonic anhydrase: blue; 66 kDa BSA: red; 443 kDa apoferritin complex: yellow) were run using identical conditions. Whereas the VNp-mNG (black) elution profile was consistent with a monomeric protein, the VNp-LZ-mNG (gray) eluted from the column in earlier fractions consistent with it existing predominantly as a dimer.

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