Gel from Scientific Research

ARPE-19ULK1GFP/GFPcells expressing FLAG-empty or FLAG-aGFP6M-PPP2CA were pre-treated with the ULK1 inhibitor MRT68921 (2 M, 2 h), where indicated, and amino acid starved with EBSS and treated with Baf-A1 (50 nM) for 2 h. For (A) and (C), extracts were resolved by SDS-PAGE and transferred onto PVDF membranes, which were subjected to immunoblotting with indicated antibodies. +AA = amino acid-rich conditions, n.s. = not significant.

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