Gel from Scientific Research

Open access visualization of Gel, Wild-type Cells, FAM83DGFP/GFPU2OS Cells, FLAG-empty, FLAG-aGFP6M-PPP1CA
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Wild-type (WT) andFAM83DGFP/GFPU2OS cells expressing FLAG-empty, FLAG-aGFP6M-PPP1CA, or FLAG-aGFP6M-PPP2CA were synchronized in mitosis using the Eg5 inhibitor S-trityl-L-cysteine (STLC) (5 μM) for 16 h. Following incubation, mitotic (M) cells were isolated through shake-off. Asynchronous (AS) cells were included as a control.

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