Gel from Scientific Research

The fate of target proteins during cell-free Trim-Away prior to proteasomal degradation was assessed in HSS using TRIM21R-R-PS. Comparison of the same endogenous and exogenous substrates targeted in Figure 1G. Samples were stopped at indicated times and resolved by SDS-PAGE and western blot analysis. Note that UFD1 is a constitutive binding partner of the targeted protein NPL4. Unmodified proteins of interest are highlighted (arrow). We assume that any bands already present at the start of the reaction are non-specific (asterisk). Longer exposures of the same western blots are shown in Figure S2.

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