Workflow from Scientific Research

Experimental pipeline for experiments in (B–D). U-2OS cells were co-transfected with a plasmid expressing BRCA1 (the WT or the variant D5) and siRNA2 to silence endogenous BRCA1, then cells were detached and seeded again. On day 5, they were treated for 3 h with bleomycin 20 μg/mL.

DOI