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Interaction of IRE1alpha LD (0–6.56 μM) with labeled IRE1alpha LD is not affected by 1 or 5 μM MANF K96A. In (C)–(E) the labeled targets were used at 20 nM. MST binding curves, showing mean ΔFnormvalues from n = 3–4 repeats per binding pair ± SEM. Kdvalues ± error estimations are indicated.
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