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Knockdown of MANF upregulates IRE1alpha. Cells were transfected with MANF small interfering RNA (siRNA) or no-targeting siRNA as a control, and protein expression was analyzed 48 h later using immunoblotting. Tubulin was used as a loading control. Mean +- SD; n = 3. Students t test;*p <= 0.05,**p <= 0.01.
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