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Estimation of the expression level of Venus constructs of MANF, N-MANF, and C-MANF for bimolecular fluorescence complementation (BiFC) assay in HEK293 cells using WB. A representative image is on the left and quantification is on the right. Venus level was normalized to alpha-tubulin level, n = 3. One-way ANOVA, Tukey's post hoc test.*p <= 0.05; ns, not significant.

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