The rate of lipid transfer from donors to acceptors, as the average change of donor volume over the course of the transfer event. Measurements were done three-dimensionally in live cells imaged by time-lapse FM of LipidTOX signals (Videos S8-S10). Live FM started 2 h after doxycycline-induced Cidec and Cidec-SUMOstar expression, and 24 h after doxycycline-induced Cidec-EGFP expression. Lipid transfer rates of individual events are represented by dots, color coded according to experimental repeat. Black lines represent median with IQR (Cidec-EGFP, 1.12 m3/h, IQR 3.18 m3/h, n = 104 from five independent experiments each analyzing 4-43 cells; Cidec-SUMOstar, 4.25 m3/h, IQR 4.42 m3/h, n = 76 from three independent experiments each analyzing 18-36 cells; Cidec, mean 17.57 m3/h, IQR 14.62 m3/h, n = 88 from three independent experiments each analyzing 23-34 cells). Only donors involved in active lipid transport and in contact with a single acceptor were considered.
