HeLa cells inducibly expressing Cidec-EGFP were vitrified, screened for LD interfaces by cryo-FM, thinned by cryo-FIB milling, and imaged by cryo-ET. The shape of the LDs is deformed where two LDs are closely apposed. The different observed morphologies are classified as follows: indentation of the larger LD caused by the smaller LD. virtual slices through tomograms acquired at areas where LDs are in close proximity (Videos S1S4). Dashed squares indicate areas corresponding to magnified images in third column. Segmentation models of LDs and membranous organelles in proximity to LDs. Pink and magenta shades, LDs; yellow, outer mitochondrial membrane (OMM); green, inner mitochondrial membrane (IMM); turquoise, ER; blue, other membranous organelles (Other). Close-ups of interfaces between LDs. Note that these are different virtual slices than in the first column. Close-ups of monolayers at the interface (fourth column) and outside the interface (fifth column). The third and fourth panels are derived from the same virtual slice. Scale bars, 150 nm in first column, 100 nm in third column, 25 nm in fourth and fifth columns.
