Microscopy from Scientific Research

FM of fixed HeLa cells inducibly expressing Cidec-EGFP (green). LDs were stained with LipidTOX Deep Red dye (magenta). (Upper) HeLa cells induced with doxycycline for Cidec-EGFP expression. (Lower) HeLa cells in the absence of doxycycline induction. Cells were fixed 24 h after the addition of oleic acid and doxycycline. The plot shows mean LD volumes per cell. Dots represent individual cells, color coded according to experiment. Black lines correspond to the median of all cells with IQR (-Dox, n = 69, from three experiments, median 0.34 m3, IQR 0.39 m3; +Dox, n = 67, from three experiments, median 1.30 m3, IQR 1.04 m3).

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