Gel from Scientific Research

Pull-down analysis demonstrating co-purification of YFP fused to full-length or truncated PpRIC from extracts of protonemata displaying estradiol-induced expression of these proteins together with GST-tagged GTP-locked PpROP1Q64L purified from E.coli. 1318 amino acids (aa), full-length PpRIC; 1228 aa, PpRIC N-terminal fragment including CRIB domain; 204318 aa, PpRIC C-terminal fragment including CRIB domain; 204228 aa, PpRIC CRIB domain (see Figure 3C). Protonemal extracts and proteins co-purified from these extracts together with GST-PpROP1Q64L or with free GST were analyzed by immunoblotting using -GFP or -GST (loading control) antibodies. The experiment was repeated three times with consistent results.

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