Gel from Scientific Research

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Endosomal vesicles from MDA-MB 231 cell were resolved in 3%–15% Optiprep gradient by ultra-centrifugation. A total of 10 fractions from top to bottom were collected and subjected to immunoblot analyses with antibodies against NMHC IIA, IIB, actin, and a variety of endosomal markers as indicated.
#Gel#Endosomal Vesicles#Cell Fractionation#Immunoblot Analysis#NMHC IIA#NMHC IIB#Actin#Endosomal Markers
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