Gel from Scientific Research

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Western blot analysis showing that SPPL3 mediates B3GNT5 cleavage and subsequent degradation by the lysosome. Bafilomycin A1 (BafA1) and chloroquine were used to inhibit the lysosome. Full-length (FL) B3GNT5 protein is readily detected in MEC and SNU878 SPPL3-deficient cells. The cleaved form of the B3GNT5 protein is readily detected in control cells with lysosome inhibition. Accumulation of LC3 was used as indicator for lysosome inhibition. n = 3.
#Gel#Western Blot#SPPL3#B3GNT5#Lysosome#Bafilomycin A1#Chloroquine#LC3#MEC SPPL3-deficient cells#SNU878 SPPL3-deficient cells
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