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Schematic showing the N-linked glycosylation process in the Golgi apparatus. N-linked glycosylation is initiated at the ER to generate a high-mannose type of glycans, followed by subsequent modification by Golgi apparatus-localized glycosyltransferases, including MGAT1 and MGAT2 (red), to form complex glycans. Kifunensine disrupts N-glycosylation by blocking α-mannosidase I activity. Removal of glycan in glycoproteins is resistant to Endo H after modification by α-mannosidase II.

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