Workflow from Scientific Research

Open access visualization of Workflow, Illustration, Single-Cell Genetic Perturbations, hBVOs, CROP-seq
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DOI: 10.1016/j.cell.2025.03.037

DOI

Schematic of single-cell genetic perturbations in hBVOs using CROP-seq. Doxycycline-inducible Cas9 iPSC line (iCRISPRn) transfected with pooled gRNA lentiviral libraries targeting 16 receptors, 18 transcription factors (TFs) (3 gRNA/gene), and 8 non-targeting gRNAs.

#Workflow#Illustration#Single-Cell Genetic Perturbations#hBVOs#CROP-seq#Cas9 iPSC Line#gRNA Lentiviral Libraries#Receptors#Transcription Factors#Non-Targeting gRNAs

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