Boxplots showing the comparison of expression levels between genes, whose promoters are covered by different length of H3K4me3 domains in hPGC samples of both sexes at indicated stage. Expression levels were represented as FPKM. For male, Wk8–10, Wk15 and Wk23 hPGC samples are represented as early, middle and late stage, respectively; for female, Wk8–10, Wk14 and Wk21 hPGC sample are represented as early, middle and late stage, respectively. Soma, gonadal somatic cells, F female, M male, w/ with, w/o without. Student’s t test is performed to examine the significant statistical difference between two groups of data in boxplots in ( f , h and j ). * P < 0.05, ** P < 0.01, *** P < 0.001, n.s. not significant.