Line Plot from Scientific Research

SK-BR-3 cells genome-edited for EGFR-mEGFP and stably expressing clathrin-mScarlet-I were treated with control siRNA or siRNA targeting HER2 (HER2-KD). The cells treated with siRNA targeting HER2 were transiently transfected with the siRNA-resistant wild-type HER2 or the HER2-GS mutant and then imaged at the bottom surfaces by TIRF microscopy. EGF was added at 120 s of the time-lapse imaging. The relative numbers of fluorescence spots of EGFR-mEGFP that appeared at the plasma membrane (left panel, n = 49, 50, 29, and 30 cells) and the relative enrichment of EGFR-mEGFP fluorescence in CCSs (right panel, n = 43, 49, 28, and 30 cells) during EGF stimulation are shown. Error bars show means SEM.

DOI