Microscopy from Scientific Research

Live-cell single-molecule imaging and tracking of endogenous (en) EGFR-Halo and HER2-Halo molecules at the plasma membrane of SUM159 cells genome-edited for EGFR-Halo/HER2-mEGFP or HER2-Halo/EGFR-mEGFP (labeled by JFX 650 -HaloTag ligand). Shown are representative single frames and tracking traces of time-lapse series acquired in the cells treated without or with EGF (100 ng/mL) by TIRF microscopy.

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