Box Plot from Scientific Research

SUM159 cells genome-edited for EGFR-SNAP were transiently expressed with HaloTag-tagged CD86, HER2, HER2-GS, EGFR, and EGFR-GS, labeled by JFX 650 -SNAP-tag ligand and JFX 549 -HaloTag ligand, and then imaged by TIRF microscopy. Shown are the relative fractions of tracks in Halo channels that interact with the SNAP-tagged endogenous EGFR ( n = 36–40 cells from 2 independent experiments). Scale bars, 5 μm. Error bars show means ± SD except for the MSD-Δt plots (means ± 95% confidence interval).

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