Left: representative flow cytometry profiles measuring the proportion of cells with DNA repair by HR (GFP + cells) after transfection with WT, p.Ala69Leufs*18 and p.Gln229* mutants of MCMDC2 among HEK293 cells with a GFP-based I-SceI-cleavable reporter. d, k, Representative data from n = 3 biological replicates. Data are shown as means ± s.e.m. Two-sided t -test was used to determine significance. The asterisk refers to P < 0.05 compared with WT. SET, SET domain; ZNF_C2H2, zinc fingers, C2H2 type; HLH, helix-loop-helix DNA-binding domain; MCM, MCM P-loop containing nucleoside triphosphate hydrolase domain; AAA-lid, AAA-lid domain found in MCM proteins.
