Percent of cells GFP positive after editing with pSFFV-GFP (HBB) or N-terminal GFP fusion (RAB11A) constructs in human K562 myeloid leukemia cells. xHDRTs were produced by treatment with the indicated amount of psoralen and UV exposure; 0 µM, isopropanol precipitated plasmid HDRT (no UV, no psoralen). The significance of experimental conditions versus 0 µM control is displayed above columns (* P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001; NS, not significant). Exact P values reported, respectively, from left to right at the HBB locus are 0.04039, 0.00050, 0.00011, 0.00018, 0.00005, 0.00015, 0.00018, 0.00074 and 0.00102. Exact P values reported from left to right at the RAB11A locus are 0.02078, 0.00029, 0.01525, 0.07793, 0.00151, 0.00253, 0.31316, 0.00211 and 0.20532.
