Pseudovirus entry efficiencies in HEK293T cells transiently expressing P.abrACE2 of wild-type and Y560K HKU5-19s S VSV and wild-type HKU5-33s S VSV. Pseudoviruses were either used untreated or after incubation with 100 g/mL trypsin for 15 min at room temperature before addition of trypsin soybean inhibitor to stop the reaction. Mock: VSV pseudovirus produced without transfecting an S construct. Each bar represents the geometric mean of three biological replicates with geometric SD, and each dot represents the geometric mean of three technical replicates. See also Figures S3,S4,S5, and S8 and Table S1.
