PCA representation of 6,000 cells (3,000 of each condition) in Fe + (blue) and Fe - (orange) conditions of raw gene expression data, data following scPrisma’s cyclic filtering (Silhouette score before/after filtering = 0.088/0.136, Calinski and Harabasz score before/after filtering = 600.802/1000.44) and enhancement, and data following filtering by Cyclum (Silhouette score = 0.052, Calinski and Harabasz score = 337.629), Seurat (Silhouette score = 9.868 x 10 - 4, Calinski and Harabasz score = 1.29 x 10 - 10) and ccRemover (Silhouette score = 9.815 x 10 - 6, Calinski and Harabasz score = 6.25 x 10 - 13).
