Biolayer interferometry analysis of the P.abrACE2 ectodomain binding to the HKU5-33s (G) RBDs immobilized on streptavidin (SA) biosensors. Binding avidities were determined by steady-state kinetics and are reported as apparent affinities (KD,app). The vertical dotted lines indicate the transition between association and dissociation phases. One representative out of two biological replicates with independently produced batches of proteins is shown for (G).
