Line Plot from Scientific Research

Temporal requirement for netB and unc-5 function during development, showing sequential gene activities taking place before symmetry breaking. Data represent the frequency of adult SYM phenotype following temporally restricted netB and unc-5 loss-of-function. Solid red and green line curves represent local regression of the frequency data obtained with 2 different methods: either ON > OFF/OFF > ON RNAi expression (data plotted as red and green circles) or using 24 h pulse RNAi expression (data plotted as red and green crosses). 20 brains are analysed for each data point and for both methods (see text, source data file and Methods for details). Shaded areas around solid black line curves represent 95% confidence interval. dPF, days post-fertilisation (25 °C); hrs APF, hours after puparium formation (25 °C). Source data are provided as a Source Data file.

DOI