Domain requirement and phenotype of Netrin pathway genes. A collection of Gal4 lines were used to drive expression of RNAi lines targeting Netrin pathway genes. Confocal brain images illustrate the expression pattern of each Gal4 line. Scale bars, 30 m. unc-5 and fra but not netB are required in H-neurons. Bars represent frequencies of ASYM (blue) and SYM (green) phenotypes in adult flies. Black triangles represent control SYM frequencies for each set of experiment. n represent the number of brains analysed. P -values are calculated with Pearson’s Chi-squared test and Benjamini and Yekutieli multiple comparison correction. P -values are: wildtype vs NetBΔ: 3,31E-08; wildtype vs unc-5 MI05371: 3,31E-08; wildtype vs Elav>NetB RNAi: 3,01E-08; wildtype vs Elav>unc-5 RNAi: 3,01E-08; wildtype vs Elav>fra RNAi: 1,48E-03; per>GFP vs per>NetB RNAi: 3,01E-08; per>GFP vs per>unc-5 RNAi: 3,01E-08; per>GFP vs per>fra RNAi: 3,94E-03; 72A10 ∩ VT017124 > GFP vs 72A10 ∩ VT017124 > unc-5 RNAi: 3,70E-04; 72A10 ∩ VT017124 > GFP vs 72A10 ∩ VT017124 > fra RNAi: 1,36E-04. Significance threshold are: *<0.05; **<0.01; ***<0.001. P -values in yellow correspond to comparisons between Gal4 controls and mutant control. Source data are provided as a Source Data file.
