Read stack of native RNA transcripts within SP1380 (M1 UK ) ssrA to speA regulon as determined by ONT long-read sequencing. Y -axis refers to the number of individual reads with each bar running along the horizontal referring to RNA read-position (and total length) relative to the ssrA-speA region (SP1380 genome coordinates 1,006,535 to 1,008,346 bp). Location of predicted speA transcriptional terminator (T) is indicated (coordinates 1,008,326 to 1,008,346 bp). RNA reads extending beyond the predicted ssrA terminator (transcriptional read-through) are coloured blue. The inset above displays RNA transcripts that extend past the ssrA terminator. A total of 0.7% of the RNA reads spanned within ssrA into speA transcript.
