Quantitative real-time PCR determining speA mRNA expression levels in 5448 (M1 global ), HKU488 (M1 global ), SP1380 (M1 UK ), SP1448 (M1 UK ), SP1380 rofA* (three rofA SNPs repaired), SP1380 ssrA* (single ssrA SNP repaired), SP1448 rofA* (three rofA SNPs repaired), SP1448 ssrA* (single ssrA SNP repaired) and 5448 ssrA* (single ssrA SNP introduced). Data from at least three biological replicates are presented as mean values +/- SD (n = 5). Statistical significance was assessed using one-way ANOVA with Tukeys multiple comparisons post hoc test (SP1380 vs. SP1380 ssrA* * p = 0.0395, SP1380 rofA* vs. SP1380 ssrA* ** p = 0.0037, SP1448 vs. SP1448 ssrA* *** p = 0.0003, SP1448 rofA* vs. SP1448 ssrA* * p = 0.0403).
