Functional assessment of amino acid substitutions of residue K26 in HIV-1 Vif. Left, amino acid mutants at Vif residue K26 were assessed for their ability to prevent packaging of A3G into virions; top, virion incorporation of A3G; bottom, amount of virus (p24 gag ) in the corresponding virion preparation. Below is a greyscale heatmap of relative A3G incorporation normalized to p24 gag based on two replicate transfections (with the exception of K26Y), with the amount of A3G in the 'No Vif' control set to 1.0 (darkest shading). Controls were run on the same gel as the samples. For Source data, see Supplementary Fig. 1 . Right, logo plot of amino acids found in the consensus of all HIV-1 clades, as well as SIVcpz (black bar) and all other SIV strains with equal distribution of each SIV (white bar). WT, wild type.
