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Anti-FLAG ChIP-qPCR assays using two different clones (CL15 and CL53) of 786-O FLAG-HIF2alpha cells compared with parental cells that were infected with a sgAAVS1 control guide. Cells were treated with 2 uM PT2399 or vehicle for 72 h prior to ChIP-qPCR with primers specific for the putative HREs for the indicated ERVs. EglN3 served as a control. Data were normalized to the untreated sgAAVS1 cells (n= 10).

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