Microscopy from Scientific Research

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Immunofluorescence of LC3. A549 and H460 cells were treated with EOAI (2 M) for 24 h. Cells were then incubated with LC3 primary antibody (1:200, overnight at 4) and Alexa Fluor 488 Goat Anti-Rabbit IgG (H + L) secondary antibody (green) (1:500, 2 h at room temperature), respectively. The nuclei were stained by DAPI (blue) (5 g/mL, 20 min at room temperature). Images were captured using fluorescence microscopy (magnification: 200). Representative images were shown.
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