Scatter Plot from Scientific Research

Axillary lymph nodes were harvested from 12-week-old Foxp3-EGFP or wild-type (WT) rats, and single-cell suspensions were gated by SSC and FSC on lymphocytes followed by the identification with mAbs of major cell populations; TCR + cells (TCR + CD4 + , TCR + CD8 + ), TCR - cells (TCR - CD4+ and TCR - CD8+), and CD161 high in total cells for NK cells. These populations were then analyzed for FOXP3 expression by EGFP expression and by using an anti-FOXP3 mAb. For both A and B , upper panels with contour plots representative from one animal and lower graphs the mean and SEM of all animals analyzed. Student’s t test * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001

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