Left y axis: fold-change in percent of IFN-γ+and/or TNF-α+CD8+T cells upon candidate peptide re-stimulation (normalized to DMSO), tested across n= 14 healthy donor PBMCs. Right y axis: fold-change in IFN-γ ELISpot spot forming units upon re-stimulation with the test peptide (versus DMSO). Each dot represents mean value across n= 3 technical replicates for a single healthy donor. Red text, HLA-A*02:01 MART-1 positive control peptides. Negative control peptide is a predicted nonbinder to HLA-A*02:01. p values calculated for each candidate peptide (versus neg10mer_4 peptide) using Wilcoxon signed-rank test (*p.adj ≤ 0.05). Red and blue asterisks: peptides with statistical significance from ICS and IFN-γ ELISpot, respectively.
