Rapid kinetics of post-mitotic transcriptional re-activation. Expression of both eppk1 ( f ) and 100537515 ( g ) is more rapidly re-activated and reached a transcriptional plateau in the post de novo transcription cell cycle of EVL cells. Normalized intensity in all cells ( eppk1 , n = 55 in the cell cycle 13, 0 time point indicating 4 hpf; n = 36 in the cell cycle 14, 0 time point indicating 4.5 hpf. 100537515 n = 24 in the cell cycle 14, 0 time point indicating 6 hpf; n = 36 in the cell cycle 15, 0 time point indicating 9.5 hpf) at each time point across de novo expression (cell cycle 13 for eppk1 , and cell cycle 14 for 100537515 ) and post-mitotic transcriptional re-activation (cell cycle 14 for eppk1 , and cell cycle 15 for 100537515 ) cell cycles. Sum of puncta 1 and puncta 2 for one cell transcriptional activity at each time point. Of note, the non-synchronized transcription of 100537515 inter-alleles in cell cycle 14 can be used as a control for the synchronized transcription of eppk1 inter-alleles in the same cell cycle, and vice versa. Data were traced from Additional file 1 : Fig. S8f, g and S9c, d for original data.
