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A schematic of time-lapse imaging of eppk1 and 100537515 gene expressions in different cell cycles of developing embryos. About 70 image stacks were recorded every 2 min during cell cycle 12–13 from 3.3 to 6 hpf, or every 5 min during cell cycle 14 from 4 to 10 hpf, and cell cycle 15 from 9.5 to 13 hpf. The image stacks were 0.4 m z -step distance and then were analyzed by maximum intensity projection.

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