Microscopy from Scientific Research

Representative images of dPspCas13b-EGFP tracking de novo transcription of eppk1 with Cy3- geppk1s , recorded every 2 min from 4 to 6 hpf during EVL of cell cycle 13, see also Additional file 7 : Movie 3. Green arrowheads indicate puncta 1, referred to as the first detected initial transcription site in each cell throughout the study, and magenta arrowheads indicate puncta 2, the later initial transcription site in each cell throughout the study.

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