Microscopy from Scientific Research

Open access visualization of Microscopy, Chemically Modified gRNA, CRISPR-dCas13, RNA Labeling, CRISPR-dPspCas13b System
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DOI: 10.1186/s13059-023-02848-6

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Chemically modified gRNA improves CRISPR-dCas13 in RNA labeling. Representative images of CRISPR-dPspCas13b system-labeled 48× GCN4 with gGCN4 (no modification, c ), MS- gGCN4 ( d ), Cy3- gGCN4 ( e ), and Cy3+MS- gGCN4 ( f ) at 6 hpf in live embryos, respectively. White arrowheads indicate labeled signals ( c–f ) and colocalization signals between Cy3 and EGFP ( e, f ).

#Microscopy#Chemically Modified gRNA#CRISPR-dCas13#RNA Labeling#CRISPR-dPspCas13b System#48× GCN4#gGCN4#Live Embryos#Cy3#EGFP

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