Microscopy from Scientific Research

Open access visualization of Microscopy, smFISH, CRISPR-dPspCas13b System, 48× GCN4, Fixed Embryos
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DOI: 10.1186/s13059-023-02848-6

DOI

smFISH confirms the CRISPR-dPspCas13b system targeting 48× GCN4 at 8 hpf in fixed embryos. White arrowheads indicate colocalization of GCN4 smFISH, Cy3- gGCN4 , and dPspCas13b-EGFP. White arrows indicate non-specific signals. Blue box, zoomed in i .

#Microscopy#smFISH#CRISPR-dPspCas13b System#48× GCN4#Fixed Embryos#GCN4 smFISH#Cy3- gGCN4#dPspCas13b-EGFP

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