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Open access visualization of Illustration, Transcriptional Activation, MPH, MS2-P65-HSF1, CMV Promoter
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Schematic of transcriptional activation constructs. MPH, MS2-P65-HSF1 complexes controlled by CMV promoter. dgRNA, nuclease-dead single guide RNA, driven by U6 promoter, harboring MS2-loop to recruit MS2-P65-HSF1 complexes and a 14-bp target sequence, which inactivates SpCas9. dgRNA 1-6, representing six dgRNAs driven by its own U6 promoter in tandem.

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